Nucleolar maturation of the human small subunit processome | Science …
We report the high-resolution cryo–electron microscopy structures of maturing human small subunit (SSU) processomes at resolutions of 2.7 to 3.9 angstroms. These structures reveal the molecular mechanisms that enable crucial progressions during SSU processome maturation.
Capturing eukaryotic ribosome dynamics in situ at high resolution
1 day ago · S3–S8, with the peptide chain connecting with A-site tRNA, represent intact eukaryotic ribosomal translocation and are well sorted according to the positions of P-site and E-site tRNA (Fig. 2a ...
Ribosome biogenesis in disease: new players and therapeutic …
Jan 9, 2023 · The eukaryotic ribosome includes two subunits, the 40S small subunit, and the 60S large subunit, which combine to form the 80S ribosome with translational activity. 8 The 40S subunit is ...
mRNA reading frame maintenance during eukaryotic ribosome
Nov 29, 2023 · We demonstrate how the accuracy of eukaryotic translocation relies on eukaryote-specific elements of the 80S ribosome, eEF2 and tRNAs. Our findings shed light on the mechanism of...
ZNF692 organizes a hub specialized in 40S ribosomal subunit …
Here, we report the identification of the zinc finger protein 692 (ZNF692), a MYC-induced nucleolar scaffold that coordinates the final steps in the biogenesis of the small ribosome subunit.
Conformational switches control early maturation of the eukaryotic ...
Jun 17, 2019 · Here we show that stable rRNA domains of the small ribosomal subunit can independently recruit their own biogenesis factors in vivo. The final assembly and compaction of the small subunit processome requires the presence of the 5’ external transcribed spacer RNA and all ribosomal RNA domains.
90S pre-ribosome transformation into the primordial 40S subunit
Sep 18, 2020 · The formation of eukaryotic ribosomes is a complex process that starts with transcription of a large precursor RNA that assembles into a large 90S preribosome, which matures to finally give the 40S small subunit of the ribosome.
Probing small ribosomal subunit RNA helix 45 acetylation across ...
Jun 2, 2022 · NAT10 is an essential enzyme that catalyzes N4-acetylcytidine (ac4C) in eukaryotic transfer RNA and 18S ribosomal RNA. Recent studies suggested that rRNA acetylation is dependent on SNORD13, a box C/D small nucleolar RNA predicted to base-pair with 18S rRNA via two antisense elements.
Crystal Structure of the Eukaryotic 40S Ribosomal Subunit in …
Dec 23, 2010 · The eukaryotic small ribosomal subunit (40 S) plays a central role in this process; it binds initiation factors that facilitate scanning of messenger RNAs and initiation of protein synthesis.
An emerging mechanism for the maturation of the Small Subunit ...
The biogenesis of the eukaryotic ribosome is a tightly regulated and energetically demanding process involving more than 200 ribosome assembly factors. These factors work in concert to ensure accurate assembly and maturation of both ribosomal subunits.
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