
PiggyBac transposon system - Wikipedia
The PiggyBac (PB) transposon system employs a genetically engineered transposase enzyme to insert a gene into a cell's genome. It is built upon the natural PiggyBac (PB) transposable …
piggyBac Transposon - PubMed
The piggyBac transposon was originally isolated from the cabbage looper moth, Trichoplusia ni, in the 1980s. Despite its early discovery and dissimilarity to the other DNA transposon families, …
The PiggyBac (PB) transposon is a mobile genetic element that efficiently transposes between vectors and chromosomes via a "cut and paste" mechanism.
piggyBac is a flexible and highly active transposon as compared …
Given its flexibility for molecular engineering and its relatively high transposition activity as demonstrated in this study, piggyBac is a promising nonviral vector for mammalian …
PiggyBac-ing Through the Genome Editing Field - Addgene
May 31, 2016 · The piggyBac™ technology can be used for numerous applications, including transgenesis, gene-trap screens, and gene editing. How does the piggyBac™ system make it …
A selectable all-in-one CRISPR prime editing piggyBac ... - Nature
Nov 12, 2021 · Here, we show that piggyBac prime-editing (PB-PE) allows for sustained expression of prime-editors. We demonstrate proof-of-concept for PB-PE in a newly designed …
Structural basis of seamless excision and specific targeting by
Jul 10, 2020 · We present cryo-EM structures of piggyBac transpososomes: a synaptic complex with hairpin DNA intermediates and a strand transfer complex capturing the integration step.
Applications of piggyBac Transposons for Genome Manipulation …
Transposons are mobile genetic elements in the genome. The piggyBac (PB) transposon system is increasingly being used for stem cell research due to its high transposition efficiency and …
piggyBac transposase tools for genome engineering - PubMed
Jun 13, 2013 · Here, we describe modified versions of piggyBac transposase that have potentially wide-ranging applications, such as reversible transgenesis and modified targeting of …
PiggyBac Transposon | System Biosciences
For transgenesis that’s easy, consistent, and not limited by cargo size, SBI’s PiggyBac Transposon System is an excellent choice. The system consists of a PiggyBac Vector and the …
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