Mar 1, 2007 · In this protocol, Steps 1–9 describe the basic method of the in vitro scratch assay for measuring migration of cell populations. In Steps 10–13, the method is adopted to track migration of...

In this study, we demonstrate an optimized method for testing the migration effect, e.g. with the naturally occurring proteins Human- and Bovine-Lactoferrin and their N-terminal peptide Lactoferricin on the epithelial cell line HaCaT.

Feb 13, 2019 · We quantify the migration velocity of scratch assays for the different cell types using the MEV method. We determine the global optimal window by calculating the objective function for the 24 scratch assays.

May 5, 2025 · Learn the materials, methods, and procedures for seeding cells, creating a consistent scratch, imaging cell migration over time using microscopy, and analyzing wound closure rates. Includes tips for optimization and controlling proliferation.

This protocol details the use of live-cell imaging to monitor collective cell migration with a scratch assay. The Omni or Lux3 can be used to image scratch closure over time.

Apr 29, 2025 · Scratch wound assays are pivotal for investigating cell migration, a crucial process in biological phenomena such as tissue repair and cancer metastasis. This technique provides insights into how cells move and interact with their environment.

Mar 4, 2025 · Discover the fundamentals of the cell scratch assay for studying cell migration. Learn best practices, experimental setup, and data analysis techniques to improve your research on wound healing and cancer metastasis.

Aug 8, 2018 · In this study, we demonstrate an optimized method for testing the migration effect, e.g. with the naturally occurring proteins Human- and Bovine-Lactoferrin and their N-terminal peptide Lactoferricin on the epithelial cell line HaCaT.

One of the most fundamental, accessible, and widely used methods in the lab is the in vitro scratch assay. It’s a powerful technique that, despite its simplicity, offers profound insights...

Scratch assay quantifies cell migration by a carpet of cells (so 100% confluent), make a scar in the middle of the carpet with pipetting (B) and see how long it closes. This method is used in particular for tumor cells, for research on cancers and cellular invasions.