Escherichia coli BL21 (DE3) is a commonly used protein production strain of the E. coli bacterium. This strain combines several features that allow for excessive expression of heterologous proteins.

BL21 (DE3) Competent E. coli is a widely used T7 expression E. coli strain. Chemically competent E. coli cells suitable for transformation and protein expression. CAUTION: This product contains DMSO, a hazardous material. Review the MSDS before handling. STORAGE AND HANDLING: Competent cells should be stored at -80°C.

Thermo Scientific BL21 (DE3) Competent Cells are suitable for the expression of non-toxic heterologous genes. The strain contains the lambda DE3 prophage that carries the gene for T7 RNA polymerase under control of a lacUV5 promoter, allowing expression of the T7 RNA polymerase to be induced with IPTG.

Jan 11, 2016 · BL21 (DE3) is suitable for expression from a T7 or T7-lac promoter or promoters recognized by the E.coli RNA polymerase: e.g. lac, tac, trc, ParaBAD, PrhaBAD and also the T5 promoter.

One Shot BL21 (DE3) Chemically Competent E. coli cells are ideal for use with bacteriophage T7 promoter-based expression systems such as pRSET and pET. This strain had been engineered to carry the λDE3 lysogen that contains T7 RNA polymerase …

Thermo ScientificTM BL21(DE3) Competent Cells are suitable for the expression of nontoxic heterologous genes. The strain contains the DE3 lysogen that carries the gene for T7 RNA polymerase under control of a lacUV5 promoter, allowing expression of the T7 RNA polymerase to be induced with IPTG.

Learn about BL21 (DE3) chemically competent cells for protein expression. This derivative for expression of nontoxic proteins features the high transformation efficiency phenotype with the gene encoding endonuclease I inactivated.

The BL21(DE3) competent cells are an all-purpose strain for high-level protein expression and easy induction. The BL21(DE3)pLysS competent cells provide tighter control of protein expression for expression of toxic proteins and are resistant to chloramphenicol.

Oct 28, 2024 · The strain contains DE3, a λ prophage carrying the T7 RNA polymerase gene under control of the lac UV5 promoter and lacIq. IPTG is required to induce expression of the T7 RNA polymerase.

BL21 (DE3) is a chemically competent E. coli cell suitable for transformation and high level protein expression using a T7 RNA polymerase-IPTG induction system. This product contains genetically modified organisms (GMO).