The autophagy research in electron microscopy - PMC
Here, we review morphological studies of autophagy involving electron microscopy. Keywords: Electron microscopy, Autophagic flux, CLEM, Immuno-gold, Cryo-EM. In the late 1950s, …
Monitoring autophagy by electron microscopy in Mammalian cells
In this chapter we describe the fine structure of early and late autophagic compartments in mammalian cells. Instructions are given for the preparation of samples for conventional …
Investigating mitochondrial autophagy by routine transmission electron ...
Oct 1, 2020 · Perhaps one of the most rigorous methods to clearly visualise mitochondria undergoing autophagic engulfment and degradation, is transmission electron microscopy …
Methods in Mammalian Autophagy Research - Cell Press
Microautophagy involves inward invagination of lysosomal membrane, which delivers a small portion of cytoplasm into the lysosomal lumen. Macroautophagy (simply referred to as …
The autophagy research in electron microscopy | Applied Microscopy …
Nov 6, 2019 · Here, we review morphological studies of autophagy involving electron microscopy. Autophagy, a highly conserved process of eukaryotic cellular recycling, plays an important role …
Next-Generation Electron Microscopy in Autophagy Research
Electron microscopy (EM) led to the discovery of autophagy in the 1950s and has been a central part of its characterization ever since. New capabilities in single particle EM studies of the …
Monitoring and Measuring Autophagy - PMC - PubMed Central …
The conventional method for electron microscopy results in clear double-membraned structures, easily identified as autophagosomes, with a wide cleft between the outer and inner …
Structure and activation of the human autophagy-initiating …
May 29, 2025 · Cryo-electron microscopy (cryo-EM) structure of a 2:1:1 stoichiometric ULK1C core. The ordered core of ULK1C was purified in a form consisting of the FIP200 N-terminal …
Seeing is believing: the impact of electron microscopy on autophagy …
Autophagy was first discovered by transmission electron microscopy more than 50 years ago. For decades, electron microscopy was the only way to reliably detect autophagic compartments in …
How to Visualize Autophagy by Microscopy - Bio-Techne
Autophagy is a recycling process that relies on the formation of a unique organelle termed an autophagosome. An elegant way to monitor autophagy is through various microscopy …