The EMBO Journal24d
Cytosolic CRISPR RNAs for efficient application of RNA-targeting CRISPR-Cas systems
Here, we show that U1 promoter-driven CRISPR RNAs (crRNAs) increase the efficiency of various applications, including RNA knockdown and editing, without modifying the Cas13 protein effector. We ...
The EMBO Journal24d
Cytosolic CRISPR RNAs for efficient application of RNA-targeting CRISPR-Cas systems
This month's cover highlights the article Immune cells adapt to confined environments in vivo to optimise nuclear plasticity for migration by Helen Weavers and Tua Karling. The cover features confocal ...
eLife5y
Global donor and acceptor splicing site kinetics in human cells
These findings are consistent with evolutionary pressure for donor sequences optimized for fast splicing. After donor site recognition by U1 snRNP, the Prp28 RNA helicase mediates the exchange of U1 ...
Frontiers6y
The Epigenetics of Alzheimer’s Disease: Factors and Therapeutic Implications
Alzheimer’s disease (AD) is a well-known neurodegenerative disorder that imposes a great burden on the world. The mechanisms of AD are not yet fully understood. Current insight into the role of ...
eLife7y
Dynamics and consequences of spliceosome E complex formation
E complex is the earliest spliceosome precursor in which the 5' SS and BS are defined. Definition occurs by U1 small nuclear ribonucleoprotein (snRNP) binding the 5' SS and recognition of the BS by ...