Hundred microliters of Matrigel (BD Biosciences; stock mixed 1:1 with ice-cold PBS and supplemented with bovine plasma fibronectin to a final concentration of 50 μg/mL) was transferred to a transwell ...

B) Schematic diagram of a RepliGut ® model cultured on a biomimetic scaffold within a Transwell insert. This produces an intestinal epithelial barrier. C) Schematic overview of fluidic flow ...

T84 WT cells were grown as a polarized monolayer on transwell inserts. Apical or basolateral surface proteins were biotinylated by addition of cell non-permeable reactive NHS-biotin to the apical or ...

Then, 3x10 3 cells from each region were resuspended in MTEC/Plus medium, mixed 1:1 with growth factor-reduced Matrigel, and seeded into Transwell inserts. MTEC/Plus was added to the lower chamber and ...

The apical line could also be used to measure the TEER across a 3D tissue model cultivated on an insert with a semipermeable membrane. The TEER measurements were obtained using one gold wire from the ...

It would be readily possible to establish a similar co-culture model in Transwell inserts and conduct the same assays. The claim that this model is the first to recapitulate the interaction between ...

Department of Health Sciences, Boston University, Boston, MA, United States C2C12 cells were from ATCC and used within the first 15 passages. C26 mouse adenocarcinoma cells were obtained from the ...

Using an approach previously described by Holder et al.(29) in which the cells are seeded on the reverse side of a transwell insert membrane and the inserts are placed in an inverted position into the ...

Briefly, a Transwell insert was coated with collagen for 1 h at room temperature, and ECs were then seeded at a density of 2×10 5 /well in a final volume of 400 μl endothelial growth medium-2 with ...