Evotec's insights into GPCRs and waveRAPID technology reveal new opportunities in drug discovery, focusing on orphan ...

The running buffer for all immobilizations assays was 50 mM Tris pH8, 250 mM NaCl, 0.05% Tween 20, 0.1 mM DTT, and 2% DMSO. All measurements of direct binding in SPR experiments were collected using ...

coli-based refolding for pMHC complex production. Furthermore, high-throughput SPR assays are employed to assess TCR-pMHC binding interactions, ensuring specificity and function for downstream ...

SPR sensograms of antibody–antigen binding; kinetics curves for the HRP-catalyzed reaction; conventional ELISA and protein G immobilized antibodies for HRP capture; the table comparing assay signals ...

From drug discovery to protein research, the MST assay is revolutionizing how ... and flexibility are essential. Methods like SPR often require immobilization of molecules, which may interfere with ...

In vitro bioassays for antibodies can be grouped into two main categories: Antigen-binding assays include ELISA, flow cytometry, surface plasmon resonance (SPR), and biolayer interferometry (BLI).

Surface plasmon resonance is a real-time and label-free technique that offers low sample consumption and high throughput when compared to other biophysical techniques and has been widely adopted in ...

Designing and performing assays that accurately measure the binding kinetics of biomolecular interactions ... basic assay design on both bio-layer interferometry (BLI) and surface plasmon resonance ...

Designing and performing assays that accurately measure the binding kinetics of biomolecular interactions ... whether you are a beginner or long-year experienced scientist working on a SPR or BLI ...