PEI-oligonucleotides transfection prior to the viral inoculation impaired HBV infection in HepG2-NCTP cells. Importantly, the PEI/oligonucleotide complex also induced the decline of HBsAg in ...

This study aimed to encapsulate plasmid DNA encoding the TP53 tumor suppressor gene (pEGFP-N1-TP53) within zeolitic imidazolate framework-8 (ZIF-8) MOFs and ZIF-8-PEI. Subsequently, the transfection ...

The empty pLKO.1 vector was used as a negative control. ACSS2 wildtype and K271R mutant Flag-tagged expression plasmids were transfected into ACSS2 knockdown 3T3-L1 cells using PEI transfection ...

Polyethylenimine (PEI) has a well-established history as a gold standard transfection reagent for both in vivo and in vitro gene transfer (Zakeri, 2018). PEI, a polycationic polymer, forms positively ...

The use of polyethylenimine (PEI) as a stable cationic polymer is gaining prominence for several small- and large-scale transient expressions in mammalian cells drives transfection reagents and ...

Transfection is a powerful analytical tool enabling studies ... PLGA NPs with polyethyleneimine (PEI) utilizing acetyl derivative (Andersen et al., 2010). Yu et al. used Solid Lipids (SLNPs) for the ...

The four delivery methods are robotic microinjection, polyethylenimine (PEI), cationic liposome (cLipo), and calcium phosphate nanoparticles (CaP). Among the four methods, microinjection has exhibited ...

Both the non-targeting control and sgKSR1 were transfected into HCT116, HCT15, and SW480 cells using PEI transfection as described previously (Longo et al., 2013). The GFP-positive cells were sorted ...

The dissolved PEI and glucose were filter sterilized. Because the efficiency of PEI transfection depends on the ratio of phosphate in the DNA to the nitrogen in the PEI (N/P ratio), the different ...

Importantly, biocompatible PEGylated MNP-PLGA-PEI NPs under an external magnetic field enhanced transfection efficiency (>7%) of plasmid DNA encoding GFP in primary hippocampal neurons compared to NPs ...

In most cases, transfection reagents that are used in small- to large-scale virus production include calcium phosphate, cationic lipids, and polyethylenimine (PEI). Calcium phosphate appears to be ...

TGE with PEI In the last decade, advances in the development of transfection reagents have emerged from different sources—all with the aim of overcoming some of the key limitations of TGE ...