Excessive sequestration of KEAP1 into phase-separated p62 bodies under conditions of impaired autophagy drives persistent ...

(F) OE/KD-CILP group Nrf2 immunoprecipitated from chondrocytes with anti-ubiquitination antibodies. (G) Western blot of Nrf2 in nucleus and cytoplasm of chondrocytes in OE/KD-group. Significant ...

Real-Time Quantitative PCR (RT-qPCR) and Western Blotting. Quantification of NRF2 activation (and/or NRF2 knockdown efficiency) on protein and mRNA levels was done by western blotting and RT-qPCR ...

The primary antibodies used for western blotting were presented below: Nrf2 (66504-1-lg, Proteintech), Akt (60203-2-lg, Proteintech), HO1 (66743-1-lg, Proteintech), SOD2 (66474-1-lg, Proteintech). The ...

In Huh7 cells, Nrf2 expression and ROS level were found increased after incubation with EPI by western blot and flow cytometry assay. But with the combination of EPI and CPT, inhibition of Nrf2 could ...

Expression of Keap1, Nrf2, PHB2, and LC3I/LC3II was analyzed by Western blot. Immunofluorescence staining was performed for colocalization with neurons (Figure I in the online-only Data Supplement).

F. Analysis of NRF2 ubiquitination in absence and presence of TRIM16 deletion variants by co‐IP assays using transiently transfected plasmid constructs as indicated. G. Western blot analysis of ...

Rorschach would use his patients' interpretations of each blot to help him evaluate their mental state. [Seeing Things On Mars: A History of Martian Illusions] The researchers had 23 first-year ...

Real-time quantitative PCR and western blot analysis showed that NRF2 positively regulates sirtuin 1 (SIRT1) at the mRNA and protein levels via the negative regulation of p53. The self-renewal and ...

Taken together, using Western blotting, ELISA and qPCR approaches coupled with Nrf2 (−/−) mice, our study clearly shows for the first time that DHA/EPA would induce Nrf2 signaling pathway and that ...

Figure 2A shows that SF administration after ICH significantly enhanced Nrf2 protein in the nuclear fraction. Figure 2. A, Western blot of Nrf2 protein in the nuclear extract from hematoma-affected ...