Other purification protocols and polishing steps are available and adjusted to the project needs. For expression we mostly use BL21(DE3) T1R pRARE2, but several other strains are used when necessary.

An E. coli outbreak linked to romaine lettuce ripped across 15 states in November, sickening dozens of people, including a 9-year-old boy in Indiana who nearly died of kidney failure and a 57-year ...

Wild-type Escherichia coli BL21 (DE3) was grown in 1 L of LB medium. After 3 hours, cells were harvested by low-speed centrifugation (6000g, 6 min) and resuspended in Buffer A (50 mM Tris-HCl pH 7.8, ...

To generate LPS-free OMVs displaying Neo-2/15 on the surface (CC-Neo215), the plasmid was transformed into the BL21(DE3) ClearColi strain via the heat shock method. The transformed E. coli containing ...

HONOLULU (HawaiiNewsNow) - The U.S. Army Garrison said testing is underway after E. coli bacteria was detected during a routine drinking water sample in the distribution system at the Tripler Army ...

In this study, we aimed to produce 3′-SL using E. coli and performed a detailed screening and comparison of six different strains: BL21(DE3), BL21star(DE3), JM109(DE3), DH5α, MG1655, and BW25113.

Share on Pinterest New E. coli outbreak in 18 states linked to organic carrots. Here’s what to know about symptoms, recalled brands, and how to reduce your risk. Grace Cary/Getty Images Organic ...

An outbreak of E. coli has led to the recall of bagged carrots from multiple brands. Across the country, dozens of cases have been confirmed, including eight in Washington state, and it’s ...

E. coli, listeria and salmonella bacteria have been making news, again, for sickening Americans. But what exactly are these tiny troublemakers? Truthfully, unless you have a microscope and samples ...

An outbreak of infections linked to McDonald’s Quarter Pounders has a lot of people across the United States concerned about their exposure to dangerous E. coli bacteria. It is not yet known ...

The strain of E. coli linked to McDonald’s Quarter Pounders is one of the leading causes of foodborne illness in the U.S. On Oct. 22, the Centers for Disease Control and Prevention announced ...