Following AAV infection, this RNP mix was electroporated (NEPA21, Nepagene) into fertilised C57BL/6 zygotes with the respective concentrations: 200 ng/μL Cas9, 100 ng/μL each guide (Table 1). These ...

CRISPR/Cas9 ribonucleoproteins (RNPs ... Because this strategy bypasses cell culture, the iPB-RNP method offers a solution to common limitations in genome editing, such as genotype dependence and ...

Department of Biotechnology and Food Engineering, Technion - Israel Institute of Technology, Haifa 32000, Israel The Russell Berrie Nanotechnology Institute, Technion - Israel Institute of Technology, ...