Deanna earned their PhD in cellular biology from McGill University in 2020 and has a professional background in medical writing. They are an associate science editor at The Scientist. View Full ...

View Full Profile. Learn about our Editorial Policies. “We have found that Cas9-mediated HDR frequencies can be increased by rationally designing the orientation, polarity and length of the donor ...

The Cas9 endonuclease is guided by a single guide RNA (sgRNA) to a specific DNA sequence, where it creates a double-strand break. This break can then be repaired through either non-homologous end ...