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A Port Vale supporter wrote: "Celebrating a season and a job well done, you’re trying to banter a 2nd place trophy in a season where Bradford got someone’s nan's plate for coming 3rd." Another ...
Most cell biology experiments result in mixed or unpurified protein samples extracted from cell or tissue lysates. The Bradford assay (or Coomassie dye–based assay) has been a standard protein ...
Cell pellets corresponding to each 15 cm plate were incubated at 4 °C in 1:10 (w/v ... protease inhibitor minitablet (Roche)) and quantified using Bradford assay (Bio-Rad). Equal amounts of protein ...
Charles River’s HCP-ELISA kit is designed to increase the efficiency and efficacy of HCP assay development. Charles River Laboratories’ enzyme-linked immunosorbent assay (ELISA) kit is designed for ...
Plates were gently shaken for 5-10 min ... The supernatant containing whole protein was collected and measured for protein concentration by the Bradford assay. Equal amounts of each sample were loaded ...
CenNLS NLuc-expressing cells, either transiently transduced or stable cell lines, were seeded in white opaque 96-well plates (Greiner ... β-Galactosidase and Bradford Assays. β-galactosidase assay was ...
3. This protein extract was then quantified by Bradford assay (Bradford, 1976). The assay was performed directly in 96-well microtiter plates. 4. GUS assay reaction buffer was prepared and 50 μl was ...
who developed the Bradford protein assay, a process that is still being used in laboratories around the world to detect proteins in tissue samples. “It made life a lot simpler.” One of the ...
Acarbose, 5-caffeoylquinic acid, sodium potassium monobasic, sodium potassium dibasic, glucose, sucrose, maltose, hexokinase glucose assay reagent, Bradford reagent ... were performed on a Pherastar ...
Position in multiwell plate Edge effects brought about by differences ... For instance, normalization to total protein content using a Bradford assay not only controls the variation in total ...
3 µl (BSA in ddH 2 O) on LVis plate in duplicates Absorbance spectrum 240-400 nm, analysis of A 280 The Bradford assay makes use of an absorbance shift of Coomassie Brilliant blue G-250 from 460 ...
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