Using spinning-disc confocal microscopy, the team followed cells in V. cholerae biofilms as they moved, disassembled and dispersed. The real-time, single-cell imaging revealed that the bacteria start ...

Fungal and bacterial biofilm-contact lens models and susceptibility to contact lens care products. Assessment of sub-clinical corneal inflammation with confocal microscopy. Assessment of bacterial ...

The resulting 3D biofilms were imaged with confocal microscopy, and the identification of donor, recipient, and transconjugant cells was performed based on the detection of green, red, and overlapping ...

This paper addresses a growing demand for an objective, fully automated method of biofilm structure description with standardized parameters that are independent of user input. In this study, we used ...

Bacterial biofilm formation in the food industry is ... Typhimurium DSB using advanced microscopy techniques, including confocal laser scanning, transmission electron, and scanning electron ...

Bacterial biofilms are complex, adaptable multi-cellular architectures that play important roles in human and environmental health and disease. Confocal microscopy has long been a core pillar of ...

Figure 2: Real-time LALFILM PRO development over time with confocal laser scanning microscopy (Source: INRA-LALLEMAND). At 12h, the positive biofilm morphology is flat, homogenous, thick, and cohesive ...

Biofilm forming capacity of the FAP1 was characterized by scanning electron microscopy and confocal laser scanning microscopy. FAP1 exhibited biofilm-related traits such as the production of ...

Confocal laser-scanning microscopy (CLSM) is the bedrock of the microscopic visualization of biofilms. Previous applications of CLSM in biofilm studies have largely focused on observations of ...

Biofilms, bacterial communities of cells encased by a self-produced matrix, exhibit a variety of three-dimensional structures. Specifically, channel networks formed within the bulk of the biofilm have ...

(Kolenbrander and London, 1993). Common techniques used in understanding biofilm architecture include scanning electron microscopy (SEM), atomic force microscopy (AFM), transmission electron ...