This study identifies 53BP1 as an interaction partner of GMCL1 (a likely CUL3 substrate receptor). The study seeks to link this finding to regulation of the mitotic surveillance pathway and paclitaxel ...

4′-Pentyl-4-biphenylcarbonitrile, a nematic liquid crystal (5CB), was obtained from Sigma-Aldrich (no. 328510). Poly(vinyl alcohol) (PVA) was sourced from Sigma-Aldrich (no. P8136). The green dye ...

Samples were then washed 4 times with 1X TBS, 5 min each washing. Secondary antibody incubation was performed in TBS 1X plus 1% BSA (Sigma-Aldrich; Cat #9048-46-8) for 2h at room temperature. The ...

To determine fatty-acid-supported respiration, Seahorse medium was supplemented with 100 µM palmitate dissolved in 1% free-fat acid-BSA (Sigma-Aldrich, cat. Num. A8806) while other substrates (i.e., ...

Briefly, 48 hours after transfection, cells were serum starved overnight with 0,25% BSA (BSA, Sigma-Aldrich, Catalog No. A9576) and 1% Fetal Bovine Serum (FBS, Gibco, Catalog No.

Briefly, 96-well polystyrene plates were pre-coated with 10µg/ml methylated BSA (Sigma-Aldrich) in PBS for 3 hours at 37°C. The plates were washed with PBS and coated with calf thymus dsDNA (10µg/ml ...

A protein stock was prepared via the dilution of a solution of bovine serum albumin (BSA, Sigma Aldrich ®, #A7284) in TE buffer. Stock concentrations were determined using the NanoDrop One ...

A protein stock was prepared using a diluted solution of bovine serum albumin (BSA, Sigma Aldrich ®, #A7284) in TE buffer. The NanoDrop One spectrophotometer was used to determine stock ...

The sections were washed with TBS-BSA and positive staining was detected using 3,3′-diaminobenzidine (DAB) (Sigma-Aldrich, St. Louis, MO, United States) for a period of 1.5 min. Then, sections were ...