C&EN18d
Efficient Biosynthesis of Sialyllacto-N-tetraose a by a Metabolically Engineered Escherichia coli BL21(DE3) Strain
State Key Laboratory of Food Science and Resources, School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China ...
C&EN25d
Novel Insights into the Control of
Key Laboratory of Plant Protection Resources and Pest Management of Ministry of Education, College of Plant Protection, Northwest A & F University, Yangling, Shaanxi Province 712100, China ...
eLife26d
Inhibiting NINJ1-dependent plasma membrane rupture protects against inflammasomeinduced blood coagulation and inflammation
Recombinant proteins were expressed using LPS free E. coli strain ClearColi BL21(DE3) (Lucigen Corporation, Cat#60810) at 37 °C for 4 hours with 500 μM IPTG after OD600 reached 0.6-0.8. Bacteria were ...
eLife2y
Structural insight on the mechanism of an electron-bifurcating [FeFe] hydrogenase
This paper describes a high resolution cryo-EM structure of an [FeFe] hydrogenase purported to operate via an electron bifurcating mechanism. The study aims to resolve a controversy regarding the site ...
Frontiers3y
Characterization and Evaluation of a Novel Conserved Membrane Antigen P35 of Mycoplasma synoviae
The TGA codon contained in CDS was changed to TGG in the DNA sequence sent for synthesis. E. coli pGro7/BL21(DE3) cell (BJBALB, Beijing, China) was used for protein expression. For the solubility test ...
Frontiers4y
Identification of Theaflavin-3,3’-Digallate as a Novel Zika Virus Protease Inhibitor
2017; Nitsche, 2019) (Figure 1A). The ZIKVpro enzyme was expressed in E.coli BL21(DE3) and then purified by a His-trap excel column (GE healthcare). SDS-PAGE analyses revealed a protein band of ...