CRISPRware is a new tool scientists can use to design the best guide RNAs to edit genes in many organisms, without the need for deep bioinformatics expertise. By making gene editing more precise and ...

New research has found a novel target with therapeutic potential for metastatic eye melanoma—an aggressive eye cancer—with ...

OptE is predicted to be a transmembrane effector protein that binds cyclic nucleotides. optSE potently restricted phage replication but mutant phages that had loss-of-function mutations in anti-CBASS ...

Enter the anti-CRISPR protein AcrVIB1, a promising inhibitor whose exact function has remained a mystery—until now. A research team from the Helmholtz Institute for RNA-based Infection Research (HIRI) ...

While most known anti-CRISPR proteins block steps along this path such as crRNA binding or target recognition, AcrVIB1 adopts a radically different strategy: Rather than blocking the binding of ...

The CRISPR-Cas system is a powerful gene-editing tool widely used in bacteria and archaea to defend against the invasion of foreign DNA. To balance this potent defense mechanism, some bacteria and ...

More information: Peter Fineran, Phage anti-CRISPR control by an RNA- and DNA-binding helix–turn–helix protein, Nature (2024).

Anti-CRISPR proteins were first characterized by Joseph Bondy-Denomy in a paper published in 2012, when he was a doctoral student in Davidson’s lab (Nature, 2012 DOI: 10.1038/nature11723).

Anti-CRISPR proteins in biotechnological applications Certain phages have evolved small inhibitor proteins known as anti-CRISPRs to inactivate bacteria’s use of CRISPR effectors for immune defense.

Anti-CRISPR proteins that inhibit Cas9 have been used in experimental settings to reduce CRISPR's "off-target" effects.