The optimal concentration depends on your target sequence, PCR type, and detection method; however, a common range is 100 to 900 nM for primers and 50 to 250 nM for probes.

Fig. 1: PCR schematic showing the amplification of the target sequence of DNA (green) from the template DNA (blue). In cycle 1, primers bind to each strand of the template (blue) and DNA polymerase ...

Multiplex PCR is an advantageous technique used in PCR applications to amplify multiple targets in a single reaction. As useful as it is, this technique presents a new set of challenges that further ...

Multiplex PCR: Multiple primers are used to sequence multiple target DNAs in the same reaction mixture. Assembly PCR: Small fragments of DNA are used to generate large DNA oligonucleotides.

We also analyzed a region of human L1 transposable elements. These L1 data provide accurate methylation patterns for the complementary strand of each repeat sequence analyzed. Hairpin-bisulfite PCR ...

The RS-PCR method relies on the generation of a first-strand cDNA template using a primer with a linker sequence, X, at the 5′- end such that synthesis of second-strand cDNA incorporates the X ...