Figure 4. Optimal growth media for plasmid DNA isolation from E. coli cells. E. coli DH5α cells were grown overnight, shaking at 37 °C, in a 2 ml, square-well, 96-well plate in either Lysogeny ...

Enhancing productivity with the Thermo Scientific™ KingFisher™ PlasmidPro Maxi Processor—a complete walk-away maxi-scale plasmid purification Introducing automated high-throughput Applied Biosystems™ ...

Plasmid DNA was purified from both 0.5 mL and 1 mL overnight cultures in either TB or LB medium. (A) Plasmid DNA concentrations and (B) A260/A280 and A260/A230 absorbance ratios were measured ...

The process begins with DdmE binding to a very short DNA fragment, known as guide DNA, which enables the protein to recognize a target plasmid and snag it by creating a bubble formation using one ...

Thermo Scientific™ KingFisher™ PlasmidPro prepares high purity plasmid DNA in approximately 75 minutes, half the traditional process time and without the need for centrifugation WALTHAM, Mass ...