In this video from Thermo Fisher Scientific, a few tips for better PCR primer design are presented. PCR, Polymerase Chain Reaction, is a common and vital part of genetic studies, biomedical research ...

The video above has some helpful tips for designing PCR primers for one purpose - cloning with restriction enzymes. PCR or Polymerase Chain Reaction, is a very common tool used in molecular biology to ...

This results in unintended products. “Primer design is both an art and a science. Not only is the right primer concentration important, but also having the right primer sequence,” says Hunter.

Some of the important design considerations for a primer used in multiplex PCR are primer length, melting temperature, primer specificity, and control of the formation of primer dimers.

Principles of TOCE assay design and signal generation during real-time PCR. (A) TOCE assay is initiated with hybridization of upstream and downstream primers (DPO) and Pitcher to the selected ...

Design primers with 40-60 percent GC content to enable primer specificity, stability, and optimal amplification. The annealing temperature should be about 5°C lower than the Tm of the primer pair to ...

Attoplex Inc., a company specializing in in vitro diagnostic (IVD) medical devices, announced on the 19th that it has signed a memorandum of understanding (MOU) with Professor Minsu Kim's research ...

LGC’s highly- qualified molecular biologists and PCR assay design experts can work with your team to create the best qPCR assay for the target of interest and experimental conditions. LGC uses ...

A new discovery has reshaped our understanding of PCR chip technology: water vapor, not air expansion, is the primary culprit behind bubble formation in polydimethylsiloxane (PDMS)-based chips.  ...