Missing piece of DNA replication puzzle identified Date: December 19, 2009 Source: Robert Wood Johnson Medical School Summary: DNA replication is a basic function of living organisms, allowing ...

These primers allow the polymerase of the main lagging-strand (polymerase delta, light green circle) to start synthesis. The direction of DNA synthesis and RF movement into DNA is indicated by arrows.

Telomerase solves this problem by adding single-stranded TTAGGG repeats to the telomere end. As for the lagging-strand, DNA synthesis should not have a problem.

A DNA helicase, powered by ATP hydrolysis, propels itself rapidly along one of the template DNA strands (here the lagging strand), forcing open the DNA helix ahead of the replication fork.

Proceedings of the National Academy of Sciences of the United States of America, Vol. 114, No. 4 (January 24, 2017), pp. 675-680 (6 pages) The eukaryotic genome is primarily replicated by two DNA ...

Model studies measure the kinetics of release upon elongation and collision showed that release was 1000-fold too slow to support the kinetics of lagging strand synthesis (115). We next took advantage ...

In DNA synthesis in vitro, mediated by the phage-induced polymerases, there is relatively limited replication — that is, replication appears to continue only until a single-stranded template is ...

An enduring mystery of 'lagging strand' DNA replication The DNA replication process is carried out by multiple protein complexes with highly specialized functions, including the unwinding of DNA ...

Journal References: Zuanning Yuan, Roxana Georgescu, Nina Y. Yao, Olga Yurieva, Michael E. O’Donnell, Huilin Li. Mechanism of PCNA loading by Ctf18-RFC for leading-strand DNA synthesis. Science ...