Optical sectioning is the process by which a suitably designed microscope can produce clear images of focal planes deep within a thick sample. This is used to reduce the need for thin sectioning using ...

Although various optical sectioning techniques (confocal, two-photon, structured illumination, and light sheet microscopy) have been developed to address these challenges, each method adheres to ...

While confocal microscopy uses a pinhole to reject out-of-focus light to generate the optical section, a multi-photon (or 2-photon) microscope uses a pulsed infrared laser to stimulate fluorescence ...

This type of a system uses cylindrical optics to focus a laser into a narrow field in the Z dimension, thus illuminating the entire XY image plane but only a ... The result is an optical section that ...