In gene editing tests, GEENIE was used to deliver CRISPR-Cas9/gRNA as ribonucleoprotein (RNP). Sanger sequencing was performed by a third party (GenOmbio) and analyzed with Synthego’s ICE ...

Gene editing technologies have cemented their place as a valued method in the biological toolkit used by researchers around the globe.

or 20 class 2 clustered regularly interspaced short palindromic repeat (CRISPR)-associated endonuclease 9 (Cas9)/gRNA complexes. The production system is robust and scalable to support the ...

It involves using a circular plasmid with a single target sequence for the Cas9 ribonucleoprotein (RNP). During manufacturing, the Cas9-RNP binds and cleaves the Nanoplasmid, linearizing the ...

The CRISPR-Cas (Clustered Regularly Interspaced ... have reduced off-target effects. 6 GC content in the gRNA sequence between 40% and 60% elevates on-target activity because higher GC content ...

Cpf1 CRISPR knocked out genetic functions in Foxn1 ... Figure 3b shows a schematic of Cpf1 RNP delivery to a mouse embryo by electroporation.