New light-controlled CRISPR tool enhances precision in genetic research
A team of researchers at Karolinska Institutet has developed a novel tool for genetic research. The study, published in ...
Nature3y
Groundbreaking first nano molecular drill crosses cell membranes to safely deliver small molecules, biological therapeutics and nucleotide-based drugs
In gene editing tests, GEENIE was used to deliver CRISPR-Cas9/gRNA as ribonucleoprotein (RNP). Sanger sequencing was performed by a third party (GenOmbio) and analyzed with Synthego’s ICE ...
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CRISPR-Cas9 Off-Target Effects: Challenges and Solutions
The CRISPR-Cas (Clustered Regularly Interspaced ... have reduced off-target effects. 6 GC content in the gRNA sequence between 40% and 60% elevates on-target activity because higher GC content ...