eLife29d
Inhibiting NINJ1-dependent plasma membrane rupture protects against inflammasomeinduced blood coagulation and inflammation
Recombinant proteins were expressed using LPS free E. coli strain ClearColi BL21(DE3) (Lucigen Corporation, Cat#60810) at 37 °C for 4 hours with 500 μM IPTG after OD600 reached 0.6-0.8. Bacteria were ...
C&EN21d
Efficient Biosynthesis of Sialyllacto-N-tetraose a by a Metabolically Engineered Escherichia coli BL21(DE3) Strain
State Key Laboratory of Food Science and Resources, School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China ...
eLife26d
Enzymatic protein fusions with 100% product yield
For recombinant expression in E. coli, BL21 gold cells were transfected with the respective plasmids and grown in lysogeny broth medium with 50 μg/l kanamycin at 22°C. Protein expression was induced ...
Engineered E. coli could be used to produce biodegradable plastics
E. coli bacteria could be used to create biodegradable plastics, reports a paper published in Nature Chemical Biology. The ...
Global News27d
E. coli concerns prompt cheese recall in several Canadian provinces
Some cheeses are being recalled in several Canadian provinces over concerns about potential E. coli contamination. The Canadian Food Inspection Agency issued the recall alert on Friday for Morbier ...
Burlington Post29d
FOOD RECALL WARNING: Cheese sold in Canada recalled due to possible E. coli contamination
The Canadian Food Inspection Agency (CFIA) issued a food recall warning for Jean Perrin brand Morbier de Scey and Raclette de Scey cheeses due to possible contamination with pathogenic E. coli. The ...
E!26d
Hairstylist Jesus Guerrero’s Sister Weighs in on “Unanswered Questions” About His Sudden Death
Jesus Guerrero’s family are still seeking answers. Amid the heartbreak surrounding the hairstylist’s sudden death at the age of 34, his sister Gris Guerrero has shared insight on the questions ...
Medscape9d
Laboratory Diagnosis of Human Hantavirus Infection: Novel Insights and Future Potential
Future Virology. 2009;4(4):383-389. These important tools were recently reported for the serologic diagnosis of hantavirus. However, it is necessary to further investigate these new methods in ...
C&EN22d
Heterologous Biosynthesis of Prenylflavonoids in Escherichia coli Based on Fungus Screening of Prenyltransferases
CAS-Key Laboratory of Synthetic Biology, CAS Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Shanghai 200032, China University of Chinese Academy of Sciences, Beijing ...