Transfection vs transduction. Applications of transfection. References. In 1928, Griffith proposed the “transforming principle” having observed that bacterial cells could take up foreign hereditary ...

Transfection efficiency depends on a number of factors. “For most adherent cells it is standard to split them the day prior so they will be ~ 80% confluent at the time of transfection.

Optimize transfection conditions: First and foremost, it is important to choose a transfection reagent that is compatible with the nucleic acid and cell type of choice. Once selected, conduct ...

DNA and mRNA transfection are both used to introduce genetic material into cells, but they differ in the genetic material used and cellular processes initiated. This infographic, part of our In Focus ...

In this article, we discuss the fundamentals of transfection, provide an overview of some of the commonly employed methods and a sample protocol. Finally, we consider some of the key applications of ...