Flow Cytometry: Cell Analysis Speeds Up Flow cytometry entered biomedical research in immunology and cancer labs out of initial interests in separating and counting cells in a mixed population, but ...

(4) Light Scatter and Detection As a cell begins to cross the laser beam, it scatters light. Light that mostly crosses the cell is detected as forward scatter and measures the cell’s size. Light that ...

A dot plot of forward light scatter versus 90 o side scatter is a measure of cell size and cell granularity respectively, the latter being dependent upon the presence of intracellular structures that ...

As apoptosis proceeds, both forward and 90 o side scatter diminish. In necrotic death, cells swell initially, and then cellular contents are rapidly released. This is seen on the flow cytometer as an ...