Flow Cytometry: Cell Analysis Speeds Up Flow cytometry entered biomedical research in immunology and cancer labs out of initial interests in separating and counting cells in a mixed population, but ...

(4) Light Scatter and Detection As a cell begins to cross the laser beam, it scatters light. Light that mostly crosses the cell is detected as forward scatter and measures the cell’s size. Light that ...

As a consequence, there is substantial overlap in the flow cytometer forward scatter profile of platelets and their MPs, due to the limited size resolution of this technology.