The neutralization titer for qPCR-MN was defined as the highest reciprocal serum dilution necessary to achieve a 90% inhibition of the qRT-PCR signal; this endpoint was found to be in agreement with ...

This assay has a readout from a luminometer, it is more quantitative and sensitive (dilutions went up to 1:24,400). Control groups sera had no neutralization activity.

To address this challenge, Kikawa, Loes and colleagues developed a high-throughput neutralization assay to measure how well individual serum samples—the component of blood that contains ...

In the PRNT assay, four MPXV recoverees exhibited some detectable neutralizing activity. Yet, all exhibited markedly less neutralization than vaccinated donors.