A deeper look into the apparent self-defense mechanisms of these bacteriophage-resistant bacteria revealed their secret weapon: a group of enzymes called restriction endonucleases, or restriction ...

but they did not identify and isolate restriction enzymes until the late 1960s. 3 In 1970, Hamilton Smith and Kent Wilcox from Johns Hopkins University discovered “endonuclease R” (later named HindII) ...

You're not likely to hear it brought up in casual conversation, but recombinant DNA has changed the world in a relatively short period of time.

Various types of endonucleases – enzymes that can cut DNA – were already known before CRISPR-Cas9. The discovery of restriction enzymes in the early 1970s heralded a new age in molecular biology.

Learn about Brink Therapeutics as it raises $4 million to go beyond CRISPR with engineered recombinases in CAR T therapy.

Cloning DNA fragments using restriction enzymes is like flying from Seattle to New York via Phoenix. You arrive at your destination, but it takes longer than necessary to get there. Restriction sites, ...

A restriction map of the 5.3 kb insert is prepared. The recombinant plasmid is digested with the enzymes listed above in single digests, and then several combinations of enzymes are tested in double ...

Restriction enzymes are one of the most important tools in the recombinant DNA technology toolbox. But how were these enzymes discovered? And what makes them so useful? Aa Aa Aa When I come to the ...