During an unperturbed S phase, DNA synthesis is catalyzed by the assembly of the multisubunit replisome at the replication fork (RF) to promote leading- and lagging-strand synthesis. Replisome ...

Finally, the single-stranded regions of DNA at the fork are covered by multiple copies of a single-strand DNA-binding protein, which hold the DNA template strands open with their bases exposed.

Journal References: Zuanning Yuan, Roxana Georgescu, Nina Y. Yao, Olga Yurieva, Michael E. O’Donnell, Huilin Li. Mechanism of PCNA loading by Ctf18-RFC for leading-strand DNA synthesis. Science ...

Coordinated action of the leading and lagging strand replicase and cycling of the lagging strand polymerase, during Okazaki fragment synthesis: An interest throughout my career has been the mechanism ...

Proceedings of the National Academy of Sciences of the United States of America, Vol. 114, No. 4 (January 24, 2017), pp. 675-680 (6 pages) The eukaryotic genome is primarily replicated by two DNA ...

The findings visualize for the first time a molecular complex - called CTF18-RFC in humans and Ctf18-RFC in yeast - that loads a "clamp" onto DNA to keep parts of the replication machinery from ...

Their findings, published recently in Nature, demonstrated that while telomerase addressed the end-replication problem at the leading strand, another complex worked in tandem at the lagging strand. 3 ...