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Researchers from Japan have developed a cell-based reporter assay for quantification of chemical ... of cells with a methylated (silenced) TK promoter following treatment with inhibitors of ...
Variants of the promoter and exon 1 sequences were identified ... activities were assayed with the Dual–Luciferase reporter assay system (Promega). Firefly luciferase activity was adjusted ...
These include control vectors with the option of using the native phosphoglycerate kinase (PGK) promoter to replace promoters of viral origin, and plasmids for coincident reporter assays for ...
Protein assays, however, are the least facile option (for example, these assays cannot be multiplexed with reporter assays) and hence, are not discussed in this article. Multiplexing a reporter ...