Scientists Can Make Copies of a Gene through PCR With the technique called polymerase chain reaction (PCR), scientists can make multiple copies of a specific genetic sequence within DNA.

Multiplex PCR: Multiple primers are used to sequence multiple target DNAs in the same reaction mixture. Assembly PCR: Small fragments of DNA are used to generate large DNA oligonucleotides.

COMplementary Primer ASymmetric PCR (COMPAS-PCR) Applied to the Identification of Salmo salar, Salmo trutta and Their Hybrids. PLOS ONE, 2016; 11 (10): e0165468 DOI: 10.1371/journal.pone.0165468 ...

Multiplex PCR is an advantageous technique used in PCR applications to amplify multiple targets in a single reaction. As useful as it is, this technique presents a new set of challenges that further ...

Each strand of DNA includes an identifying sequence that matches a short sequence of nucleotides called a PCR primer. When the primer is added to the DNA solution, it bonds with matching DNA ...

Fig. 1: PCR schematic showing the amplification of the target sequence of DNA (green) from the template DNA (blue). In cycle 1, primers bind to each strand of the template (blue) and DNA polymerase ...

Abundance, richness, and diversity of fungi in Urtica dioica roots for the three primer pairs tested fITS7/ITS4, gITS7/ITS4, and 5.8S-Fun/ITS4-fun (Ta = 57 °C), the addition of PNA clamps (+PNA ...