This diagram illustrates the creation of recombinant DNA using a plasmid as a vector. The host plasmid is cleaved by restriction endonucleases, creating sticky ends. These sticky ends allow for the ...

Hanahan’s method for improving DNA uptake played a large part in making recombinant DNA technology accessible. “Competent E. coli have become a mainstay across the life sciences,” Hanahan noted. “It ...

Public fears of biological harm. That is the new reality brought to us by bioterrorism -- but it also was the reality of the early days of recombinant-DNA research, when scientists… ...

August 2, 2021 Plasmid DNA lies at the heart of viral and mRNA vector production because it provides the coding sequences for gene-based advanced therapy medicinal products (ATMPs).

The process begins with DdmE binding to a very short DNA fragment, known as guide DNA, which enables the protein to recognize a target plasmid and snag it by creating a bubble formation using one ...

Plasmid DNA (pDNA) is a fundamental starting material for therapeutic development, including for viral vectors, mRNA therapies, and DNA vaccines. Researchers use capture methods such as anion exchange ...

Johnson, I. S. Human insulin from recombinant DNA technology. Science 219, 632–637 (1983). Fineberg, S. E. et al. Immunogenicity of recombinant DNA human insulin. Diabetologia 25, 465–469 (1983).

Hopewell-based team delivers on ProBio's promise of speed, quality, and reliability—offering a transparent, fixed-cost model and ≥85% supercoiled plasmid DNA in record time for clinical ...

Biotechnology governance entreaties released, echoing legacy of 1975 recombinant DNA guidelines New texts housed at Rice confront evolving challenges in biotechnology oversight amid advances in AI ...

Gene editing was a new idea in the mid-1970s. So when Harvard and MIT planned new research in recombinant DNA, alarm bells went off. "People were worried about a 'Frankengene,'" says Lydia Villa ...

Kaneka Eurogentec, an FDA inspected CDMO focused on the production of injectable grade cGMP biopharmaceuticals, announced the manufacture of 1 kg of plasmid DNA, in a single batch, for a major ...