Step 2: Conjugation of the pSLICER plasmid into D. radiodurans where it expresses the codon-optimized I-SceI endonuclease that cuts at the 18-bp I-SceI restriction site within the SD cassette.

There are a wide range of potential benefits to a fully synthetic approach to minicircle production. Cell-free systems bypass plasmid amplification bottlenecks and recombination inefficiencies, with ...

Scientists at deCODE genetics/Amgen have constructed a complete map of how human DNA is mixed as it is passed down during reproduction. The map marks a major step in the understanding of genetic ...

Scientists at deCODE genetics/Amgen have constructed a complete map of how human DNA is mixed as it is passed down during reproduction. The map marks a major step in the understanding of genetic ...