The recombination process yielding the full adenoviral plasmid requires only one step, and always results in the formation of only the desired recombinant adenoviral genome.

Step 2: Conjugation of the pSLICER plasmid into D. radiodurans where it expresses the codon-optimized I-SceI endonuclease that cuts at the 18-bp I-SceI restriction site within the SD cassette.

There are a wide range of potential benefits to a fully synthetic approach to minicircle production. Cell-free systems bypass plasmid amplification bottlenecks and recombination inefficiencies, with ...

PR Newswire Wed, Jan 22, 2025, 11:00 AM2 min read REYKJAVIK, Iceland, Jan. 22, 2025/PRNewswire/ -- Complete recombination map of the human-genome, a major step in genetics ...

It has been known for some time that rAAV is able to drive levels of homologous recombination that are up to 1,000-fold higher than when using a simple plasmid. Despite this vast improvement ...

Authors on the paper in Nature: Complete human recombination maps, Kari Stefansson CEO of deCODE genetics with Bjarni V. Halldorsson and Gunnar Palsson, scientists at deCODE genetics.

Scientists at deCODE genetics/Amgen have constructed a complete map of how human DNA is mixed as it is passed down during reproduction. The map marks a major step in the understanding of genetic ...

Scientists at deCODE genetics/Amgen have constructed a complete map of how human DNA is mixed as it is passed down during reproduction. The map marks a major step in the understanding of genetic ...