To demonstrate the power of PCR–mediated recombination and provide an example ... Controls included the transfection of pNL4–3 plasmid DNA and the TA vector plasmid DNA. Approximately half ...

along with their drug-resistance plasmid as a new, improved cloning vector to show that frog DNA could also be replicated and transcribed in E. coli. While Paul Berg received the 1980 Nobel Prize in ...

It was the first drug produced through recombinant DNA technology and among the first genetically engineered products to be available to consumers. Following the success of Humulin, recombinant DNA ...

In 1973, the Cohen-Boyer team introduced a plasmid fragment from one strain of Escherichia coli, conferring kanamycin resistance in ...

The rationale behind four plasmids is to enhance safety, as separating genetic components reduces chances of recombination. The ‘transfer plasmid’ or lentiviral vector contains the transgene ...

prevent recombination of replication-competent HIV. All lentivirus vectors produced in the VDL are produced in compliance with GLP standards. The production of a lentivirus begins with cloning the ...

All our multiexpression systems (currently MultiBac, MultiMam and MultiColi) rely on in vitro Cre-loxP recombination of Acceptor and Donor ... plasmids to be recombined with a single Acceptor plasmid ...

Recombinant DNA is one of the first stories in modern biotechnology history. The National Museum of American History contains collections which trace this foundational technique of genetic engineering ...