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A plasmid is a small, circular, double-stranded DNA molecule that is distinct from a cell's chromosomal DNA. Plasmids naturally exist in bacterial cells, and they also occur in some eukaryotes ...
Major players in the bacterial & plasmid vectors market are Sigma-Aldrich Inc., ATUM, QIAGEN, Promega Corporation, Thermo Fisher Scientific, Inc., GenScript Biotech Corporation, Takara Bio Inc ...
Sample Preparation Grow bacterial clones, having plasmid DNA of interest Inoculate single clones into a 96-well, 2 mL, square-well plate holding 1.2 mL/well Terrific broth (TB) with suitable ...
96-well, silica filter-plate plasmid extraction kits can be acquired from commercial suppliers. These methods harvest bacteria cells before completing alkaline lysis.
The finding was unexpected because DdmE has similar qualities to a superfamily of Argonaute proteins that trigger their host bacteria's death to avoid plasmid invasion. Argonautes, however, are ...
Birmingham scientists have identified essential genetic code for a method called plasmid curing, which aims to ‘displace’ antibiotic resistance genes from bacteria. Plasmids, which are small, circular ...
The IncP-1 plasmid backbone adapts to different host bacterial species and evolves through homologous recombination. Nature Communications, 2011; 2: 272 DOI: 10.1038/ncomms1267 ...
Using a combination of biology and mathematics, researchers at the Max Planck Institute for Terrestrial Microbiology have uncovered a common principle of bacterial plasmid segregation. The team led by ...
It can kill bacteria by itself, but it is most often used in conjunction with other antibiotics. When bacteria, such as E.coli, are constantly exposed to colistin, those that have no defence, die.