DNA isolation and analysis week 2: Restriction enzyme digestion and electrophoretic separation Introduction Once pure samples of DNA have been prepared, it can be manipulated in several ways; cut at ...

Enhancing productivity with the Thermo Scientific™ KingFisher™ PlasmidPro Maxi Processor—a complete walk-away maxi-scale plasmid purification Introducing automated high-throughput Applied Biosystems™ ...

Plasmid DNA was purified from both 0.5 mL and 1 mL overnight cultures in either TB or LB medium. (A) Plasmid DNA concentrations and (B) A260/A280 and A260/A230 absorbance ratios were measured ...

Thermo Scientific™ KingFisher™ PlasmidPro prepares high purity plasmid DNA in approximately 75 minutes, half the traditional process time and without the need for centrifugation WALTHAM, Mass ...

1. Preparation of a cell extract: To extract DNA from a tissue/cells of interest, in this experiment the heart muscle of rats, the cells have to be separated and the cell membranes have to be ...