The "Extraction buffer" helps in carrying ... can be used to remove these contaminants, leaving the DNA in a pure form. The standard way to de-proteinize a cell extract is to add phenol or a 1:1 ...

Potential contaminants include: proteins, RNA, chromosomal DNA, non-specific PCR products, residual salts, organic chemicals (i.e. phenol, chloroform or ethanol), residual detergents, as well as ...

Set up two reactions for digestion of genomic DNA: Incubate the two reactions at 37 °C for 3 h. Extract the reactions with phenol/chloroform (1:1, v/v), recover the DNA by precipitation with ...

Cells are homogenized in guanidinium thiocyanate and the RNA is purified from the lysate by extraction with phenol-chloroform at reduced pH 1. This method allows many samples to be processed ...