at the phase interface. 5. Repeat the phenol:chloroform:isoamyl alcohol extraction. 6. Extract the sample with an equal volume of chloroform:isoamyl alcohol to remove the phenol.

2. Add 200 µL of Phenol-Chloroform to 200 µL of cell lysate in 1.5 mL tube. Vortex for 60 seconds and centrifuge at room temperature and max speed for 5 minutes. 3. Carefully extract top aqueous layer ...

Alternatively, the phenol interface can be re-extracted with 100 microlitres of TE buffer following removal of the aqueous layer. The re-extracted DNA can be added to the first extraction before the ...

Expensive commercial deoxyribonucleic acid (DNA) extraction kits are widely used for bloodmeal identification. This study assessed the performance of an inexpensive phenol-chloroform DNA extraction ...

All commercially available kits were able to shorten the duration of DNA extraction. Results With regard to detection limits, both phenol: chloroform extraction and the QIAamp DNA Mini Kit provided ...

The extraction buffer composition can be found in the Supplementary material. After samples were placed in the 1× extraction buffer they were incubated in a shaker/incubator overnight at 56°C. The ...