The deamination of the amino acids creates NH 3, a weak base, which causes the medium to become alkaline. The alkaline pH causes the phenol red indicator to begin to turn red. Since the incubation ...

When Zhao and his team replaced phenylalanine with other amino acids besides cysteine, they found that the mutants still tolerated phenol, suggesting that the trait depends on the specific position ...

Cells are homogenized in guanidinium thiocyanate and the RNA is purified from the lysate by extraction with phenol-chloroform at reduced pH 1. This method allows many samples to be processed ...