A new discovery has reshaped our understanding of PCR chip technology: water vapor, not air expansion, is the primary culprit ...

To carry out real-time PCR, researchers have to choose not only what primers to design (see Box 2) but also what detection chemistry to use. In many cases these decisions will be influenced ...

This handbook explores key strategies for optimizing qPCR workflows, from sample preparation to data analysis, helping you ...

The findings, published on 15 Feb 2025 in Frontiers of Computer Science, address a critical challenge in quantitative PCR ...

High coverage, easy assay design and assay compatibility provide a high degree of flexibility; at the same time, in silico PCR ensures high specificity of primer and probe combinations in the ...

Extension: Nucleotides are added to the primers, which serve as extension points to continue new strand synthesis. Endpoint analysis: Scientists analyze PCR products typically using agarose gel ...

The findings, published on February 15, 2025, in Frontiers of Computer Science, address a key challenge in quantitative PCR ...