This results in unintended products. “Primer design is both an art and a science. Not only is the right primer concentration important, but also having the right primer sequence,” says Hunter.

By using resources available on the Internet, you can design, test, and order PCR primers, as well as optimize PCR conditions. Primer databases and testing ...

Integrated DNA Technologies (IDT) has launched a new and enhanced version of the open access PrimerQuest® online tool, for efficient PCR primer and probe design. Selection parameters have been ...

Some of the important design considerations for a primer used in multiplex PCR are primer length, melting temperature, primer specificity, and control of the formation of primer dimers.

Unlike other highly multiplexed PCR approaches, NuProbe's SADDLE approach uses computational primer design to minimize primer-dimer formation, and thus does not require enzymatic primers and ...

Design primers with 40-60 percent GC content to enable primer specificity, stability, and optimal amplification. The annealing temperature should be about 5°C lower than the Tm of the primer pair to ...

PCR primers designed to bind within highly variable regions yield high specificity but risk high incidence of false-negative results. The answer is to design in a balance between these extremes.

Attoplex Inc., a company specializing in in vitro diagnostic (IVD) medical devices, announced on the 19th that it has signed a memorandum of understanding (MOU) with Professor Minsu Kim's research ...