To carry out real-time PCR, researchers have to choose not only what primers to design (see Box 2) but also what detection chemistry to use. In many cases these decisions will be influenced ...

The findings, published on 15 Feb 2025 in Frontiers of Computer Science, address a critical challenge in quantitative PCR (qPCR) primer design. Unlike existing software that requires manual ...

High coverage, easy assay design and assay compatibility provide a high degree of flexibility; at the same time, in silico PCR ensures high specificity of primer and probe combinations in the ...

Extension: Nucleotides are added to the primers, which serve as extension points to continue new strand synthesis. Endpoint analysis: Scientists analyze PCR products typically using agarose gel ...

The findings, published on February 15, 2025, in Frontiers of Computer Science, address a key challenge in quantitative PCR ...

LGCs consultative approach to working with you, together with an industry-leading portfolio of probes and oligo modifications gives you confidence that you are getting the best PCR assay design to ...