Nuclear localization signals (NLSs) are motifs that mediate the transport of proteins to the nucleus, but finding an NLS within your protein of interest can be tricky. In the 15 November EMBO Reports ...

The study applied a new approach that installed hairpin internal nuclear localization signal sequences (hiNLS) at selected sites within the backbone of CRISPR-Cas9.

Rearranging the genomic architecture of the nuclear localization sequence helped the system to assemble particles in the cytoplasm of producer cells but still access the nucleus in recipient cells.

The researchers have identified specific sequences in lamin A's tail region, termed "Lamin A-Characteristic Sequences" (LACS1 and LACS2) that inhibit its rapid localization to rupture sites.