The method features a set of optimal type IIP restriction enzymes whose respective restriction sites define the multiple cloning site (MCS) in the cloning vectors (Supplementary Table 1).

These restriction sites are often clustered in polylinker regions or multiple cloning sites which enable the researcher to select the most convenient restriction enzyme combination for several ...

To identify vector sequences, alignments will be prepared between your edited forward and reverse complement sequences and the sequence in the pstblue1vector.txt file. (This file contains the sequence ...

General sub-cloning or PCR cloning into the multiple cloning sites with feasible restriction site(s) to use and only involves the 20 common restriction endonucleases listed below. (List of the 20 ...

Gibson Assembly Cloning is an elegant and robust seamless cloning methodology that has been widely adopted by the scientific community and enables the assembly of multiple DNA fragments ... protein ...