As the leading strand T7 replisome does not pause during primer synthesis, this cannot explain why the leading and lagging strands are synthesized at the same rate. However, the authors found that ...

Mechanism of PCNA loading by Ctf18-RFC for leading-strand DNA synthesis. Science, 2024; 385 (6708) DOI: 10.1126/science.adk5901 Qing He, Feng Wang, Michael E. O’Donnell, Huilin Li.