As a result, downregulation of hStn1 accumulates ss G-rich DNA specifically at lagging-strand telomeres, increases telomere fragility, and delays synthesis of lagging-strand telomeres. Our ...

Sometimes the lagging strand synthesis stops, but the leading strand continues to grow. This shows up as a dark area in the glowing strand, because the dye doesn’t stick to single-stranded DNA. “We’ve ...

“Not only did it not elongate, but it also got shorter,” said de Lange. Normally for the lagging strand, DNA synthesis is unhindered, and the replisome starts synthesizing the last Okazaki fragments ...

leading-strand DNA replication should copy the CCCTAA repeats to generate the TTAGGG repeat strand, while lagging-strand synthesis should do the opposite, making new CCCTAA repeats. The end ...

Sometimes the lagging strand synthesis stops, but the leading strand continues to grow. This shows up as a dark area in the glowing strand, because the dye doesn't stick to single-stranded DNA.

Thus, a lagging-strand polymerase that does become stalled at a template ... Once the leading-strand polymerase resumes DNA synthesis, it catches up to the helicase and rapid template unwinding and ...

and another clamp loader called RFC in both human and yeast to thread the clamp onto the lagging strand. The clamp then closes and signals to the polymerases that they can begin replicating DNA.

2024.07.017]. The scientists determined that Mrc1 and Mcm2 form a co-chaperone complex, ensuring distribution of histones to the lagging strand during DNA replication. In mutants of Mrc1 ...