Overcoming the resolution limit in a light microscope of around half a wavelength of light (about 250 nanometers) is one of ...

Digital holographic microscopy's ability ... DHM has many features in common with interference microscopy techniques, which also make use of a reference wave to record the phase of optical waves.

The trapping beam is directed to the back aperture of a 100× NA=1.42 oil immersion objective via two lenses (L3, L4), linearly polarized by a half wave plate and polarizer and fed into the microscope ...